Time Kill Test 

Time Kill Test is a basic microbiology method  for assessment of Antimicrobial Activity of An Antimicrobial Test Material or Disinfectant. The Kill Time Test is carried out to evaluate the microbial reduction by a disinfectant against selected bacteria or fungi. Various organisms are studied depending upon the type of analysis and test material, however, most common organisms tested include: Staphylococcus aureus, Salmonella cholerasuis, Pseudomonas aeruginosa, E.coli, Aspergillus niger, and Trichophyton mentagrophytes.

The test product or a dilution of the test product is brought into contact with a known population of microorganisms approximately 10⁶ CFU/mL for a specified period of time at a specified temperature. At selected time points including zero time, aliquots are removed and placed into a neutralizer blank. Dilutions of the neutralizer are made and selected dilutions plated onto agar. Colonies are enumerated .

The percent or log reduction, or both, from either an initial microbial population, or test blank is calculated.

Amount of Sample Required

100 milliliters per lot per organism 

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