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ASTM E2180

Standard Test Method for Determining the Activity of Incorporated Antimicrobial Agents in Polymeric of Hydrophobic Materials

This method involves thin layer of the inoculated agar slurry onto the test and untreated control material (triplicate samples minimum).After the specified contact time (24 h commonly used), surviving microorganisms are recovered via elution of the agar slurry inoculum from the test substrate into neutralizing broth. Serial dilutions are made, plated and incubated for 48 h at a specified temperature dependent upon the optimal temperature for test organism. Bacterial colonies from each dilution series are counted and recorded. Calculation of percent reduction of bacteria from treated versus untreated samples is made.Exposure periods may be modified (up to 96 h) to address more resistant microorganisms.

Turn Around Time: Eight to ten working days (if commonly tested organisms are tested.)

Sample Requirements:

3.0 x 3.0 cm square samples of the treated and control test materials (

Number of Samples: Since each time point requires 3 test pieces, for example for time 0 hour and 24hours= 3x2 = 6 pieces will be required per organism ordered.

An untreated control sample is also required.

Test Organisms:
Staphylococcus aureus ,Pseudomonas aeruginosa or Klebsiella pneumoniae .Other microorganisms such as yeast or fungi may also be tested using this procedure.

To purchase a copy of ASTM E2180 test method visit ASTM at


Last Updated On: 07-23-09
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