Standard Test Method for Determining the Activity of Incorporated Antimicrobial Agents in Polymeric of Hydrophobic Materials
This method involves thin layer of the inoculated agar slurry onto the test and untreated control material (triplicate
samples minimum).After the specified contact time (24 h commonly used),
surviving microorganisms are recovered via elution of the agar
slurry inoculum from the test substrate into neutralizing broth.
Serial dilutions are made, plated and
incubated for 48 h at a specified temperature dependent
upon the optimal temperature for test organism.
Bacterial colonies from each dilution series are counted
Calculation of percent reduction of bacteria from treated
versus untreated samples is made.Exposure periods may
be modified (up to 96 h) to address more resistant microorganisms.
Turn Around Time: Eight to ten working days (if commonly tested organisms are tested.)
3.0 x 3.0 cm square samples of the treated and
control test materials (
Number of Samples: Since each time point requires 3 test pieces, for example for time 0 hour and 24hours= 3x2 = 6 pieces will be required per organism ordered.
An untreated control sample is also required.
Staphylococcus aureus ,Pseudomonas aeruginosa or Klebsiella pneumoniae .Other microorganisms such as yeast or fungi
may also be tested using this procedure.
To purchase a copy of ASTM E2180 test method visit ASTM at astm.org
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