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ASTM E 2149

ASTM E 2149-Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions.

This test method is designed to evaluate the resistance of non-leaching antimicrobial treated specimens to the growth of microbes under dynamic contact conditions.

The dynamic shake flasks in which all the samples are shaken in a wrist action shaker to ensures good contact between the bacteria and the treated fiber, fabric, or other substrate by constant agitation of the test specimen in a bacterial suspension during the test period.

Microbial concentrations in the treated and untreated samples are determined at zero as well as 1 or 24 hrs contact time or time specified by the sponsor.


Organisms: Klebsiella pneumoniae, ATCC No. 4352 Other organisms like Staphylococcus aureus , E.coli….. may be tested upon request.


Sample Requirements between 0.5 and 2.0 grams


Contact time: 1 hr.. unless a different contact time is specified


Turn around time: 5 to 8 days


To purchase a copy of ASTM E2149 test method visit ASTM at astm.org

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