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AATCC 100 Test Method

Assessment Of Antimicrobial Finishes On Textile Materials

AATCC 100 antimicrobial test method is a quantitative method in which assessment of antibacterial finishes is determined on the textile materials . Following exactly, full AATCC 100 test method, Accugen labs, tests the degree of antibacterial activity intended in the use of these materials .

Contact times are selected that the test material would be effective in significantly reducing microbial counts during use (generally 0, 24, and at times 48 hours).

AATCC 100 test is performed with treated and non-treated swatches of the test material.

According to AATCC 100 antimicrobial test procedure, 1.0 mL of test organism suspension at approximately 1000,000 CFU/mL is inoculated to test sample.

At selected time point, organisms are extracted in a neutralizer media which is diluted and plated accordingly.

The log reduction and or percent reduction is determined by using Organism survival data from the treated and non-treated materials at that contact time.

Organisms according to AATCC 100 Test:

Staphylococcus aureus and Klebsiella pneumoniae. Other organisms may be tested upon request.

AATCC 100 Test Sample Requirements:
Samples Size: 48 x 48 mm squares ( 1.9 plus minus 0.03 inches) or 48 x 48 mm(( 1.9 plus minus 0.03 inches)diameter circles Or Sheets can be submitted.

Untreated sample: 48 x 48 mm squares or 48 x 48 diameter circles Or Sheets can be submitted

Turn Around Time: Eight to ten working days are required if commonly used organisms like the one described in AATCC 100 test standards are tested.

Scope of AATCC 100 Antimicrobial Test Method : This antimicrobial test method provides a quantitative evaluation of the antimicrobial activity of the test material. It determines both bacteriostatic activity (inhibition of multiplication) as well as bactericidal activity (killing of bacteria) of the antimicrobial test agent.

Limitations of AATCC 100 Antimicrobial Test Method : AATCC 147 is faster antimicrobial test method for determining a qualitative residual antibacterial activity of textile materials in comparison to AATCC 100 .Besides, the nature of fabric (hydrophobic), can effect the antimicrobial activity test results by AATCC 100 test method.

To purchase a copy of AATCC 100 test method visit AATCC at


Last Updated On: 07-23-09
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